data-analysis

Confirming genotypes by visualizing mutations from RNAseq data

Kazu Nozue / 2019-01-08

RNAseq is a powerful tool for transcriptome analysis. It is also useful for genotyping (for example, 1). Here I demonstrated an example of confirmation of genotype used for RNAseq. There are some unavoidable errors due to unexpected mistakes in many steps (eg. contamination of seeds, contamination …

data-analysis

Annotating sequences by benchling.com

Kazu Nozue / 2018-12-06

Annotating sequences is critical for making a construct in molecular biology. Benchling.com is a powerful tool for automating the annotation process. This example explains how to label “2x35S promoter” region in sequences of interest. […] benchling.com plasmid annotation (step1) […] …

data-analysis

Busy dad needs project management skills!

Kazu Nozue / 2018-09-12

Here I would like to start describe briefly how I manage my work and personal projects. […] As scientists we need to convince others that your results are not by chance. Here are four advices I want to emphasize here. […] You data and lab notebooks have to be well organized for others …